辐射诱发胸腺淋巴瘤c-mye基因启动子甲基化检测
Detection on promoter methylation of c-myc gene in thymic lymphomas induced by ionizing radiation
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摘要: 目的 研究辐射诱发小鼠胸腺淋巴瘤c-mcy基因启动子CpG岛甲基化状态和mPNA表达的变化.方法 X射线照射BALB/c小鼠建立胸腺淋巴瘤模型,采用硫化测序PCR(bisulfite sequencing PCR,BCP)法和逆转录-聚合酶链反应(RT-PCR)分别检测c-myc基因启动子CpG岛甲基化状态和mRNA的表达.结果 辐射诱发胸腺淋巴瘤c-myc基因启动子CpG岛与正常胸腺组织比较呈去甲基化状态,RT-PCR检测结果显示,胸腺淋巴瘤c-myc基因mPNA相对表达量(1.12±0.99)明显高于正常胸腺组织(0.89±0.08),2组比较差异有统计学意义(P<0.01).结论 c-myc基因启动子去甲基化可能与辐射诱发胸腺淋巴瘤密切有关.Abstract: Objective To study methy lation of CpGislands in prom oter region of c-mcy gene and the change of mRNA expression in thymic lymphomas of mice induced byionizing radiation.Methods The thymic lymphom as model was made with X-ray exposure in BAL B/c mice.The methylation of CpG islands in prom oter region and the mRNA expression of c-myc gene were detected with bisulfite sequencing PCR and RT-PCR.Results CpG islands inprom oter region of c-myc gene were at demethy lation state in thymic lymhom as induced by ionizing radiation against normal thymus.The mRNA expression of c-myc gene in thymic lymphomas(1.12±0.99) was higher than that of in normal thym us tissue(0.89±0.08) with a tatistical sign if icance(P < 0.01).Conclusion There is a corre lation between promoter demethylation of c-myc gene and thymiclym phom as induced by ionizing radiation.
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