辽宁省HIV-1株的基因序列测定和亚型分析
Subtype and sequence analysis on HIV-1 strains in Liaoning province
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摘要:目的 通过DNA序列分析, 确定辽宁省HIV-1流行毒株的亚型.方法 从辽宁省HIV-1抗体阳性者中选择16例, 其中经性接触感染者8例, 经静脉吸毒和血液途径感染者7例, 垂直传播者1例, 采集全血提取DNA作为PCR扩增的模板, 套式引物扩增HIV-1前病毒DNA的C2~V3区用于测序.所测序列与国际标准株及国内外参考序列进行比较, 确定被检标本的亚型.结果 辽宁省流行的HIV-1病毒分属A、B、C、G4种亚型, 其中经性途径传播者8例分别为B'亚型和A亚型, 静脉吸毒者4皆为C亚型, 经血途径感染者分别属B'和G亚型, 垂直传播者为A亚型.亚型内基因离散率分别为: A亚型(7.5±1.0)%, B'亚型(8.5±0.9)%, C亚型(5.4±0.9)%, G亚型(13.5±2.1)%.结论 辽宁省目前HIV-1的流行株亚型较为复杂, 不同传播途径感染HIV毒株亚型有所不同, C亚型毒株只见于静脉吸毒者, 经性途径和血途径感染主要为B'亚型, 而A亚型和G亚型只见于非洲劳务输出人员及其配偶子女.Abstract:Objective To identify the HIV-1 subtype epidemic in Liaoning by nucleotide sequencing.Methods Eight of the selected 16 HIV-1 sero-positive individuals were infected with HIV-1 by sexual contacts, 7 were IDUs or blood recipients and blood donors, 1 was vertical transmitant.DNA frag ments of HIV-1 envgene were amplified by PCR from provirus in human genome.The amplified products of C2-V3 region of HIV-1 were sequenced and compared with the consensus sequence of reference HIV-1 strains and some strains epidemic in Aisa.Results HIV-1 strains epidemic in Liaoning were found to be of genetic subtype A, B., C, G.8 of sexual transmission were subtype B.and A.4 of IDUs were subtype C.Blood recipients and blood donors were of subtype B.and G, 1 of vertical transmit ant was subtype A.Conclusion There was several subtypes HIV-1 invading Liaoning province, which manifested the complexity of HIV epidemiology in Liaoning province.The HIV-1 subtype in different transmission route showed respective character: Subtype C were found only in IDUs, individuals infected HIV-1 by sexual contact and blood route were mainly of subtype B., however subtype A and G can only be found in laborer returned from Africa and his spouse and children.
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