Abstract: ObjectivesThis study investigated the long-term effect of artificial fiber complexes(AFC)on liver structure and gastrointestinal function in hypercholesterolemic rats.MethodsMale and female weanling SDstrain rats(80~100g)were divided into five groups,twelve rats per group.As the control groups, Group A and B were fed either the normal diet or high-cholesterol diet.Gr oup C.D.and E were given one of the diets incorpo rated AFC at the differ ent level of 5%、10% or 20% respectively.The experiment lasted three months.ResultsAFC affect the feed intake of the experimental rat;20% of AFC could raised fecal weight,but 5%.10% of AFC could lower it;20% of AFC could significantly raise the weight of small intestine wall,but 5%.10% of AFC could lower small intestine wall wegltt.All level of AFC significantly lowered liver weight compared with high-cholesterol group,and the weight was positively correlated with AFC levels in diets;20% AFC elevated activity of plasma GPT and GOT; Cloudy swelling and fatty degeneration of liver cells could be observed in 20% of AFC.Conclusionslong-term intake of AFC at lower levels could be beneficial for and small intestinecs function; on the other hand,higher levels were harmful to ratsc health.