内蒙古科右中旗地区蒙古、汉族人群HBV基因分型

席云峰; 苏军; 王睿; 王文瑞

doi:10.11847/zgggws2014-30-02-28

内蒙古科右中旗地区蒙古、汉族人群HBV基因分型

Genotyping of hepatitis B virus among Han and Mongolian populations in Keyouzhong banner of Inner Mongolia

摘要

摘要: 目的了解乙型肝炎病毒（HBV）基因型在内蒙古兴安盟科右中旗地区蒙古族及汉族人群之间的分布情况。方法采用S基因区的序列分析法和系统发生树分析法确定HBV基因型，HBV血清标志物检测采用ELISA法检测。结果105例蒙古族样本中93例测序成功，其中B型26例（27.96%）均为B2亚型，C型47例（50.53%）均为C2亚型，D型20例（21.51%）均为D1亚型；60例汉族样本中51例测序成功，其中B型10例（19.61%）均为B2亚型，C型32例（62.74%）全部为C2亚型，D型9例（17.65%）均为D1亚型；从NCBI中BLAST的分型结果与S基因系统发生树的分型结果完全相同。结论内蒙古兴安盟科右中旗地区蒙古族及汉族HBV基因型均为B、C、D型，B、D基因型以蒙古族为主，C基因型以汉族为主。

Abstract: ObjectiveTo investigate the distribution of hepatitis B virus(HBV) genotype among Han and Mongolian in Keyouzhong banner of Inner Mongolia.MethodsWe used sequencing and phylogenetic analysis to determine the distribution of genotypes,subtypes and S mutations of HBV.HBV serologic markers were detected with enzyme-linked immunosorbent assay(ELISA).ResultsFor 105 Mongolian,93 were sequenced successfully,with 26 genotype B2(27.96%),47 genotype C2(50.53%),and 20 genotype D1(21.51%).For 60 samples from Han,51 were sequenced successfully,with 10 genotype B2(19.61%),32 genotype C2(62.74%),and 9 genotype D1(17.65%).All genotyping results based on Basic Local Aligment Search Tool(BLAST) of National Center for Biotechnology Information(NCBI) were identical to those based on phylogenetic tree.ConclusionThe genotypes of HBV are genotype B,C and D among Han and Mongolia populations in Keyouzhong banner of Inner Mongolia,with dominant genotypes of band D in Mongolia and C in Han population.

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