Protective effect of selenium on lead-induced toxicity in rats
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摘要: 目的探讨染铅大鼠毒性作用及补硒对铅致损伤的保护作用。方法选取Wistar大鼠40只,随机分为对照组、低高剂量染铅组和硒干预低、高剂量染铅组,每组8只;对照组喂饲基础饲料,低高剂量染铅组在基础饲料中分别添加180、270 mg/kg的醋酸铅,硒干预低、高剂量染铅组分别在低高剂量染铅饲料组中添加0.5 mg/kg亚硒酸钠;7周后处死大鼠检测其体重、脏体比值、血液学、酶学变化及血铅含量等指标。结果给药7周后,高剂量染铅组和硒干预高剂量染铅组大鼠体重分别为(160.22±20.29)和(148.50±9.11)g,均低于对照组的(204.60±26.21)g(P<0.05);除硒干预低剂量染铅组外,肾、脾重量及肝、肾、脾与体重比值均受到影响;低、高剂量染铅组和硒干预高剂量染铅组血红蛋白分别为(12.11±2.06)、(10.24±1.61)和(11.97±1.39)g/dL,均低于对照组的(16.61±4.30)g/dL(P<0.05),硒干预低剂量染铅组为(13.86±2.11)g/dL,与对照组比较差异无统计学意义(P>0.05)。结论选择合适剂量的硒可以对铅致损伤产生保护作用。Abstract: ObjectiveTo study the toxicity of lead(Pb)poisoning in rats and the protective effects of selenium(Se)supplementation against the damage caused by Pb. MethodsForty Wistar rats were randomly divided into five groups:control group,180 mg/kg Pb group,270 mg/kg Pb group;180 mg/kg Pb+0.5 mg/kg Se group,and 270 mg/kg Pb+0.5 mg/kg Se group.Pb and Se were administered in the form of lead acetate and sodium selenite in the diet.The rats in the control group were given basal diet.All the rats were anesthetized and blood and organs were collected at the end of 7 weeks.Body weight,ratio of organ/body weight,hematological and enzymatic parameters and blood Pb were analyzed. ResultsThe body weight of the rats in 270 mg/kg Pb group(160.22±20.29 g)and 270 mg/kg Pb + 0.5 mg/kg Se group(148.50±9.11 g)was significantly lower than that in control group(204.60±26.21 g)(P<0.05).The ratio of organ/body weight was changed in 180 mg/kg Pb group,270 mg/kg Pb group and 270 mg/kg Pb+0.5 mg/kg Se group,but not in 180 mg/kg Pb+0.5 mg/kg Se group.The hemoglobin concentration in 180 mg/kg Pb group(12.11±2.06 g/dL),270 mg/kg Pb group(10.24±1.61 g/dL)and 270 mg/kg Pb + 0.5 mg/kg Se group(11.97±1.39 g/dL)were decreased compared with that in control group(16.6±4.30 g/dL)(P>0.05),while the hemoglobin level in 180 mg/kg Pb + 0.5 mg/kg Se group was comparable with that in control group. ConclusionSe at a suitable dose can protect against the damage caused by Pb.
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Keywords:
- lead /
- selenium /
- toxic effect /
- protect effect
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