Abstract: Objective To study the effects of targeting cluster of differentiation 147(CD147)RNA interference(RNAi)on cell proliferation,cell cycle and apoptosis of androgen-independent prostate cancer LNCaP-AI cell line.Methods The shRNA vector targeting CD147 gene was transfected into LNCaP-AI cells by Lipofectamine 2000.The stable cell line with down-regulated CD147 was obtained after G418 screening.The cell proliferation,cell cycle and apoptosis were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry,respectively.Results Compared with the control group,silencing expression of CD147 in LNCaP-AI cell significantly inhibited cell proliferation(P<0.05).The percent of G₀/G₁ phase cells increased from 69.76±3.83%to 79.40±4.02%(P<0.05),S and G₂/M phases cells decreased from 23.51±2.58%to 17.03±2.02%and from 6.73±0.70%to 3.57±0.21%,respectively(P<0.05),which showed that G₀/G₁ phase of experiment cells were arrested.There was no obvious difference in the RNAi transfected cells in apoptosis.Conclusion Downregulation of CD147 via RNAi technology could decrease the cell proliferation and induce aberrant cell cycle.