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植物花色苷对血小板凋亡通路中BCL2家族影响

Effects of anthocyanin on BCL2 family of platelet apoptosis pathway

  • 摘要: 目的 探讨花色苷矢车菊素-3-葡萄糖苷(Cy-3-g)对血小板凋亡通路中BCL2家族影响。方法 采集健康志愿者外周静脉血,制备纯化血小板悬液;纯化后的血小板与终浓度为0.5、5、50 μmol/L 花色苷Cy-3-g孵育,胶原作为凋亡诱导剂诱导血小板凋亡后,采用流式细胞仪测定血小板凋亡率,提取总蛋白用western blot 检测抗凋亡蛋白BCL-XL,促凋亡蛋白BAK、BAX、BID及cleaved BID蛋白表达量。结果 与对照组比较,5 μmol/L花色苷能够明显增加血小板凋亡率;与对照组比较,5、50 μmol/L 花色苷组BCL-XL蛋白表达水平分别为(0.739±0.047)、(0.884±0.004)明显下调,差异均有统计学意义(P<0.01);与对照组比较,5、50 μmol/L花色苷组 BAK、BAX、BID蛋白表达水平分别为BAK:(1.667±0.063)、(1.531±0.039),BAX:(2.271±0.165)、(1.895±0.018),BID:(1.861±0.231)、(1.311±0.121)明显上调,差异均有统计学意义(P<0.05)。结论 花色苷能够通过调控BCL2家族蛋白的表达水平促进血小板凋亡。

     

    Abstract: Objective To investigate the effects of anthocyanin cyanidin-3-glucoside (Cy-3-g)on BCL2 family of platelet apoptosis pathway.Methods Peripheral venous blood samples of healthy volunteers were collected and purified platelet suspension was prepared.Gel-filtered platelets were pre-incubated with 0.5,5,and 50 μmol/L Cy-3-g and induced by collagen.Flow cytometry was used to measure platelet apoptosis rate and total platelet proteins were extracted;the expressions of anti-apoptotic proteins BCL-XL and pro-apoptotic proteins BAK,BAX,BID and cleaved BID were quantified with western blot.Results Compared with the control group,5 μmol/L Cy-3-g significantly increased the rate of platelet apoptosis and 5 μmol/L (0.739±0.047),50 μmol/L(0.884±0.004)Cy-3-g effectively reduced expression of anti-apoptotic protein BCL-XL in platelets(P<0.01).Compared with the control group,5,50 μmol/L Cy-3-g significantly promoted the expressions of pro-apoptotic proteins BAK,BAX,BID and the differences were of statistic significance at concentrations of 5 μmol/L(1.667±0.063,2.271±0.165,1.861±0.231)and 50 μmol/L (1.531±0.039,1.895±0.018,1.311±0.121),respectively(all P<0.05).Conclusion Cy-3-g effectively promotes platelet apoptosis by affecting the proteins expression of BCL2 family.

     

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