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microRNA靶序列单核苷酸多态性与胃癌易感性关系

Correlation between single nucleotide polymorphism of microRNA target site and gentic susceptibility of gastric cancer

  • 摘要: 目的研究炎性相关基因IL-1F5(interleukin-1 factor 5)的3’非编码区microRNA(miRNA)靶序列单核苷酸多态性(SNP)与胃癌易感性关系。方法采用1∶1频数匹配病例-对照研究对研究对象进行基因分型,应用多因素条件logistic回归及多因素降维法(MDR)分析SNP与胃癌易感性的关系及其与环境因素的交互作用。结果rs2472188位点GC基因型(OR=1.50,95%CI=1.13~1.99)和rs2515401位点CT基因型(OR=1.42,95%CI=1.09~1.86)是胃癌的易感基因;单体型分析显示,单体型1(CCGCA)(OR=2.08,95%CI=1.27~3.40)、单体型4(CTATA)(OR=1.98,95%CI=1.48~2.66)、单体型5(CTATC)(OR=10.13,95%CI=4.43~23.16)可增加胃癌发病风险,而单体型2(CTACA)(OR=0.18,95%CI=0.12~0.28)、单体型3(CTACC)(OR=0.37,95%CI=0.23~0.59)、单体型9(GCGTC)(OR=0.39,95%CI=0.27~0.58)可降低胃癌发病风险;具有rs2472188、rs2515401以及rs2515402组合的人群是胃癌发病风险的高危人群(OR=6.17,95%CI=4.61~8.36)。结论rs2472188位点GC基因型、rs2515401位点CT基因型是胃癌的易感基因型,多位点联合作用对于胃癌的发生可能有协同作用。

     

    Abstract: Objective To assess the correlation between single nucleotide polymorphism(SNP)of microRNA(miRNA)target site and the gentic susceptibility of gastric cancer. Methods A 1:1 matched case-control study was adopted in this study.Genotyping was carried out among the subjects.Conditional logistic regression model and multifactor-dimensionality reduction(MDR)method were used to analyze the correlation between SNP of interleukin-1 factor 5(IL-1F5)and genetic susceptibility of gastric cancer.The gene-environment interaction was also analyzed. Results GC genotype in rs2472188 locus(odds ratio OR=1.50,95% confidence interval 95%CI=1.13-1.99)and CT genotype in rs2515401 locus(OR=1.42,95%CI=1.09-1.86)were susceptible genotypes for gastric cancer.Haplotype analysis showed that haplotype 1(CCGCA)(OR=2.08,95%CI=1.27-3.40,haplotype 4(CTATA)(OR=1.98,95%CI=1.48-2.66)and haplotype 5(CTATC)(OR=10.13,95%CI=4.43-23.16)increased the risk of occurrence of gastric cancer,whereas haplotype 2(CTACA)(OR=0.18,95%CI=0.12-0.28),haplotype 3(CTACC)(OR=0.37,95%CI=0.23-0.59)and haplotype 9(GCGTC)(OR=0.39,95%CI=0.27-0.58)decreased the risk. Conclusion Genotypes of GC in rs2472188 locus and CT in rs2515401 locus may be susceptible genotypes for gastric cancer.Combined effects of multigene alleles and multi-locus genotype may have a synergistic effect in the carcinogenesis of gastric cancer.

     

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