Detection and analysis of CD4+CD25+regulatory T cell in peripheral blood from newborns with HBV intrauterine infection
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摘要: 目的 探讨CD4+CD25+调节性T细胞(Treg)在乙肝病毒(HBV)宫内感染新生儿外周血中的表达和意义。方法 选择乙肝表面抗原(HBsAg)阳性孕妇及其分娩新生儿79例,用酶联免疫吸附试验检测孕妇和新生儿外周血HBV标志物,实时荧光定量PCR 检测母亲和新生儿外周血 HBV DNA 含量,用流式细胞仪检测新生儿外周血CD4+CD25+ Treg和CD4+CD25+Foxp3+ Treg水平。结果 宫内感染HBV新生儿外周血CD4+CD25+ Treg和CD4+CD25+Foxp3+ Treg占CD4+T细胞比例分别为(10.57±2.25)%和(1.67±0.37)%,均高于未感染新生儿,差异有统计学意义(P<0.05),随产妇HBV DNA载量增加,新生儿外周血CD4+CD25+ Treg和CD4+CD25+Foxp3+ Treg增加,CD4+CD25+ Treg比例高于阴性组(P<0.05);CD4+CD25+ Treg和CD4+CD25+Foxp3+ Treg均与产妇HBV DNA载量呈正相关(r=0.430、0.409,P<0.05)。结论 Treg可能通过抑制机体细胞免疫反应影响乙肝病毒清除,新生儿HBV宫内感染可能与Treg表达上调有关。
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关键词:
- 乙肝病毒(HBV) /
- 宫内感染 /
- CD4+CD25+调节性T细胞(Treg) /
- 流式细胞术
Abstract: Objective To investigate the expression and significance of CD4+ CD25+ regulatory T cells in peripheral blood from newborns with hepatitis B virus(HBV) intrauterine infection.Methods We selected 79 hepatitis B virus surface antigen( HBsAg) positive pregant women and their newborns.The HBV markers of the pregnant women and the neonates were detected with enzyme-linked immunosorbant assay(ELISA).HBV DNA contents of pregnant women and neonates were detected with real-time fluorescent quantitative-PCR(RT-PCR).The expression of CD4+ CD25+ regulatory T cells(Tregs) in peripheral blood from the newborns were detected and analyzed with flow cytometer.Results The percentage of CD4+CD25+Tregs/CD4+ and CD4+CD25+Foxp3+ Tregs/CD4+ were 10.57?2.25% and 1.67?0.37% in peripheral blood from the newborns of intrauterine infection group and were significantly higher than those of non-intrauterine infection group(P<0.05).The expression of CD4+CD25+ Tregs and CD4+CD25+Foxp3+ Tregs increased with maternal blood HBV DNA level.The proportion of CD4+CD25+ Tregs was significantly higher than that of negative group(P<0.05).The expression of CD4+CD25+ Tregs and CD4+CD25+Foxp3+ Tregs were correlated positively with HBV DNA level in pregnant mothers(r=0.430,r=0.409,P<0.05).Conclusion CD4+CD25+ regulatory T cells could inhibit the elimination of HBV through suppressing cellular immune response,and its upregulation of expression may be responsible for HBV inntrauterine infection. -
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