EGCG乙酰化衍生物对肝癌细胞细胞毒增敏作用
Chemotherapeutic sensitizing effect of peracetate EGCG on hepatocellular carcinoma cells
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摘要: 目的探讨表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)乙酰化衍生物(AcEGCG)是否对人肝癌细胞SMMC-7721有细胞毒增敏作用。方法用四甲基偶氮噻唑蓝(MTT)法检测EGCG和AcEGCG分别联合柔红霉素(DNR)对人肝癌细胞SMMC-7721的毒性作用,实验设35、100、160μmol/L EGCG及AcEGCG组、35、100、160μmol/L EGCG及AcEGCG分别联合柔红霉素DNR(0.9μmol/L)组,金正均法计算q值,判断两药相互作用。结果 35、100μmol/L AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用较相同剂量的EGCG强(67.0%对48.1%,78.9%对65.0%),差异具有统计学意义(P<0.05),160μmol/L AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用比EGCG稍弱(P>0.05)。结论在一定剂量范围内,AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用较EGCG强。Abstract: ObjectiveTo study the chemotherapeutic sensitizing effect of epigallocatechin gallate(EGCG)and peracetate EGCG(AcEGCG)on hepatocellular carcinoma cells SMMC-7721.MethodsThe SMMC-772 cells were treated with daunorubicin(DNR)and/or EGCG(AcEGCG).The cytotoxicity of the compounds was detected by 3-4,5-dimethylthiazol-2-yl2,5-diphenyltetrazolium bromide(MTT)method.ResultsAcEGCG(35.0μmol/L,100.0μmol/L)combined with DNR(0.9μmol/L)could increased the inhibitive rate of SMMC-7721 cells from 48.1% to 67.0% and from 65.0% to 78.9% for EGCG combined with DNR,respectively.ConclusionAcEGCG combined with DNR could enhance the cytotoxicity of AcEGCG on SMMC-7721 cells than that of EGCG in some given dosage.
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