Relationship between single nucleotide polymorphisms of HSP70 genes and susceptibility to hypertension in workers exposed to heat stress
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摘要: 目的 探讨HSP70基因多态性与高温作业工人高血压发病易感性关系.方法 选取某钢铁企业158名炼钢、轧钢工人为高温组,同厂的76名库工为对照组.使用统一的调查表收集个人信息并测定血压值,聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)方法分析HSP70基因多态性,运用PHASE2.0遗传分析软件计算单倍型.结果 HSP70各基因型在高温组与对照组及高血压与非高血压人群中的分布差异均无统计学意义(P>0.05).进一步将研究对象分为正常血压组、血压高值组和高血压组后发现,高温组血压高值组明显高于高血压组(P<0.05);高温组和对照组2组合并后的总人群中,具有C/C基因型的人群与具有G/G基因型的人群比较,易感血压高值的调整OR(95%CI)为3.52(1.02～12.11).高温组血压高值组HSP70-2基因G/G基因型频率明显高于高血压组及正常血压组,而HSP70-2基因G/A基因型在血压高值组的分布频率低于高血压组与正常血压组;经Logistic回归分析,高温组具有HSP70-2基因G/A基因型的人群与G/G基因型的人群比较,易感血压高值的调整OR(95%CI)为0.25(0.08～0.78).单倍型对分析,高温组具有GAT/***单倍型的人群与GAT/GAT单倍型对的人群比较,易感高血压的调整OR(95%CI)为1.29(1.02～12.44).结论 HSP70-1基因C/C基因型与人群血压高值有关联.HSP70-2基因G/G基因型与高温作业工人血压高值有关联,G/A基因型可降低高温作业工人血压高值的风险性,GAT/***单倍型与高温作业工人高血压的易感性有关.Abstract: Objective To study the relationship between HSP70 polymorphisms and susceptibility to hypertension in workers exposed to heat stress.Methods Totally 158 steel workers and rollers were selected as the exposure group and 76 workers without heat stress exposure were selected as the control group.The general status and the blood pressure were investigated.HSP70 genotypes were analyzed by using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)technique and the haplotype was caluclated with PHASE 2.0 software.Results HSP70 genotype frequencies of the exposure and the non-exposure groups were not significantly different in the workers with normal blood pressure or hypertension.HSP70-1 C/C genotype frequency in the workers with higher blood pressure was significantly higher than those in heat-unexposed workers with hypertension(P<0.05).Compared with G/G genotype,C/C genotype was a risk factor for increased blood pressure,with an adjusted odds ratio[OR]of 3.52(95% confidence interval[CI]:1.02-12.11)for all workers.HSP70-2 G/G genotype frequency in the workers with higher blood pressure in exposure group was significantly higher than that of workers with hypertension or normal blood pressure in unexposure group(P<0.05).HSP70-2 G/A genotype frequency in the workers with higher blood pressure in exposure group was significantly lower than that in wokders with hypertension or normal blood pressure in unexposure group(P<0.05).The results of multiple logistic regression analyses showed that G/A genotype in exposure group was a protective factor for increased blood pressure with an adjusted OR of 0.25(95% CI:0.08-0.78)and GAT/*** haplotype pairs were risk factors for hypertension of exposure groups,with an adjustment OR value of 1.29(95% CI:1.02-12.44).Conclusion HSP70-1 C/C genotypes are associated with increased blood pressure in the workdrs.HSP70-2 G/G genotypes are associated with increased blood pressure in workers exposed to heat stress.G/A genotype is a protective factor for increased blood pressure.GAT/＊＊＊haplotype pairs are associated with hypertension susceptibility in workers exposed to heat stress.
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Keywords:
- high temperature /
- hypertension /
- HSP70 /
- gene polymorphism
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[1] 田凤契,郑金平,孙建娅,等.焦化作业工人淋巴细胞HSP70表达及作用[J].中国公共卫生,2007,23(2):204-205. [1] WHO-ISH.Hypertension Guidelines Committee.1999 World Health Organization International Society of Hypertension Guidelines for management of hypertension[J].Hypertension,1999,17:151-183.
[2] 山西省标准局.山西省工业卫生监测监护规范[S].山西省地方标准DB 14/35-14-91,1991:146-148. [3] 杨晓波,孙建娅,梁华山,等.焦炉工HSP70基因多态性和外周血淋巴细胞DNA损伤的关系[J].环境与职业医学,2006,23(6):488-491. [4] 李瑞芳,孙建娅,张萍,等.钢铁企业高温作业工人高血压患病影响因素分析[J].中国公共卫生,2009,25(7):115-118. [5] 邬堂春,贺涵贞,张国高.热应激反应的研究现状与展望[J].中华劳动卫生职业病杂志,2000,18:365-367. [6] 李芳泽,周舫,蒋长征,等.热应激蛋白70基因多态性与急性高原反应的关系[J].中华劳动卫生职业病杂志,2004,22(6):413-415. [7] 肖成峰,李芳泽.HSP70家族基因多态性及其与疾病易感性之间的关系[J].国外医学遗传学分册,2003,26(4):205-208.
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