Abstract: Objective To evaluate the anti-oxidative effect of folate on the levels of oxidative stress in male patients with hyperlipidemia.Methods Eighty male volunteers with hyperlipidemia were randomized into intervention and control group and the intervention group was treated with folate(2.5 mg/d)for 3 months.Physical examination,epidemiologic and dietary survey,the measurements of serum folate and oxidative stress markers were conducted before and at the end of folate administration.Results There was no difference in meal nutrimental levels between the two groups before and after the intervention(P>0.05).Compared with the control group,the most of the biochemical indicators in folate group were not siginificantly different(P>0.05);whereas the serum folate and total antioxidant capacity(T-AOC)increased significantly(P<0.05)and malondialdehyde(MDA)concentration decreased significantly(t=-5.995,P<0.001);serum 8-iso-prostaglandin(8-iso-PG)level and serum glutathione peroxidase(GSH-Px)activity showed no change(P>0.05).There were significant relationships between serum folate and MDA(r=-0.536,P<0.001)and T-AOC(r=0.239,P=0.033)levels.Conclusion Folate supplementation may improve the levels of oxidative stress in male patients with hyperlipidemia.