Abstract: Objective To compare antibacterial property of seven kinds of polyurethane materials with different content of nanoAg-SiO₂.Methods Materials with nanoAg-SiO₂ at dose of 0%,0.5%,1.0%,1.5%,2.0%,2.5%,and 5.0% were prepared.Staphylococcus aureus and Escherichia coli were adopted as test strains.Sticking film method was used to determine living bacterium number and to calculate antibacterial rate.The antibacterial property of the materials was ranked.Bacteriostatic ring experiment with nanoAg-SiO₂ content of 1.0% and 2.5% was conducted simultaneously.The diameter of bacteriostatic ring was determined on 1,3 and 7 day of the test to determine antibacterial persistence of the materials.Results Antibacterial rates of polyurethane materials with nanoAg-SiO₂ content of 0.5%,1.0%,1.5%,2.0%,2.5%,and 5.0% for Staphylococcus aureus and Escherichia coli were all more than 90%.Antibacterial rate of the materials increased with the increment of nanoAg-SiO₂ concentration.Bacteriostatic ring diameters on 1,3,and 7 day of the test show ed no obvious difference(P > 0.05).Conclusion All polyurethane materials with nanoAg-SiO₂ content of 0.5%,1.0%,1.5%,2.0%,2.5%,and 5.0% have antibacterial property and lasting resistance to bacterial and could be used as antibacterial material in daily life and medical practice.