沈阳市零售肉类中弯曲杆菌污染状况调查
Investigation on contamination by Campylobacter of retail raw meat in Shenyang
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摘要:目的 了解大都市不同类型市场零售肉类弯曲杆菌污染状况。方法 2003年1月~12月每月从沈阳市超级市场、农贸市场、街边市场采集零售生肉类样品, 包括鸡肉、鸭肉、兔肉、猪肉和牛肉, 分离检测弯曲杆菌(Campylobacter spp), 分析不同肉类、不同市场类型、不同季节污染状况, 应用PCR方法鉴定弯曲杆菌种类及其血清型。结果 123份鸡肉样品中94%检出弯曲杆菌, 85份鸭肉样品受污染的占96%, 65份兔肉样品占97%, 猪肉和牛肉样品中的污染相对较少, 138份猪肉样品中占31%, 89份牛肉样品中占35%。200份肉类样品共分离到弯曲杆菌525株, 其中55%是空肠弯曲杆菌(Campylobacter jejuni), 41%是大肠弯曲杆菌(Campylobacter coli), 4%是其他类型的弯曲杆菌。结论 超市样品弯曲杆菌污染率最低, 街边市场销售的肉类污染率最高; 零售肉类弯曲杆菌污染水平高于国外相关报道, 应引起重视。Abstract:Objective To determine the prevalence of Campy lobacter in retail raw meats obtained from markets and to investigate the association of microbial contamination with product type, season, and market.Methods Retail raw meat samples were randomly obtained from three kinds of stores Shenyang from January 2003 to December 2003 and were examined for the species and the serotypes of Campy lobacter by PCR.Results The majority(94%)of 123 chicken carcasses were contaminated with Campylobacter.Approximately 96% of 85 duck carcasses and 97% of 65 rabbit carcasses yielded Campylobacter, whereas 31% of 138 pork chops and 35% of the 89 beef chops were positive for this pathogen.A total of 525 Campylobacter isolates were obtained from 200 meat samples; 55% of these isolates were C.jejuni, 41% were C.coli, and 4% were other species.Conclusion Significant differences in the microbial prevalence were observed for supermarket samples with the lowest contamination, street market samples showing the highest contamination, and farm market samples in the middle.
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